α-Hydroxyglutaric acid

What is measured?

Synonyms: 2-Hydroxyglutaric acid, α-hydroxyglutarate, 2-hydroxyglutarate. Method(s): GC-MS/MS ( 1 ).

Indication(s)

Assessment of α-hydroxyglutarate status in patients with IDH mutated malignancies.

Specimen, collection and processing

Patient/subject: No special precaution. Matrix: Serum or EDTA plasma. Volume: Minimum volume is 50 µL, but 200 µL is optimal and allows reanalysis. Preparation and stability: Probably stable.

Transportation

Frozen, on dry ice.

Reported values

Reported values: 0.15-2 µmol/L, and may be 10-fold higher in cancer patients with the IDH1/2 mutation(s). Intraclass correlation coefficient (ICC): na.

Literature

1 . Midttun, Ø., McCann, A., Aarseth, O., Krokeide, M., Kvalheim, G., Meyer, K., and Ueland, P.M. (2016). Combined measurement of 6 fat-soluble vitamins and 26 water-soluble functional vitamin markers and amino acids in 50 μL of serum or plasma by high-throughput mass spectrometry. Anal Chem 88, 10427-436. 2 . Ježek, P. (2020). 2-Hydroxyglutarate in Cancer Cells. Antioxid Redox Signal, 33, 903-926.